Modulation of oxidative stress, inflammatory and apoptotic response by curcumin against cerebral ischemia reperfusion injury in a mouse model

Abstract

BackgroundIschemic stroke, which is a direct result of a decrease in blood flow to the mind parenchyma, is the most widely recognized reason for stroke. ObjectiveThis assessment was endeavored to examine the neuroprotective effect of curcumin in cerebral ischemia-reperfusion harm in mice model. Materials and methods40 adult male Swiss albino mice (17–18) weeks of age were used randomly and were divided into four groups (10 mice in each): Group (1) sham group: In this group, the mice did not undergo a carotid occlusion. (2) control group (stroke group): the mice in this group underwent bilateral common carotid artery occlusion for 30 min. Tracked by two-hour reperfusion. Group (3) vehicle group: Mice in this group received distilled water to dissolve curcumin 30 min before the operation, then 30 min of ischemia Bilateral common carotid artery occlusion BCCAO, followed by reperfusion for 2 h. Group (4) curcumin treatment group: mice received 100 mg/kg curcumin intraperitoneal (IP). With bilateral common carotid artery occlusion for 30 min. Then reperfusion for 2 h. ResultsToward the finish of the investigation the degree of the IL-6,NF-kb,Mcp-1 Bax and F2IsoP were altogether expanded in the contro1 group as compere with sham group while fundamentally decline the degree of Bcl-2 in the control group as compere with sham group. Treatment with curcumin essentially decline the degree of IL-6,NF-kb,Mcp-1 Bax and F2IsoP in the curcumin-treated group as compare with control group while there is a critical increment in the degree of Bcl-2 in the curcumin-treated group as compared with control group. Histopathological examination showed that curcumin altogether improve the seriousness of cerebral damage in the mice experienced regular carotid artery occlusion. ConclusionAfter effects of the investigation discovered that 1nflammatory cytokines, apoptosis pathways and oxidative stress are engaged with brain ischemia incited by mutual regular carotid artery block. Brain ischemia-reperfusion damage able to be changed by curcumin by means of its mitigating, hostile to apoptosis and against oxidant impacts.