Abstract
1. 1. Pure erythrocytes preparations, free from platelets and white cells, were incubated for a long time without hemolysis. 2. 2. Dose-response experiments performed with (a) cardioglycosides (ouabain and K-strophantoside), (b) steroid hormones and their glucuronides (tetrahydrocortisol, oestradiol and the respective 3-glucuronic derivatives) and (c) ouabain-like compounds purified in our laboratory (0.7 kDa and 2–4 kDa respectively) emphasise a modulatory effect [activation of Na + efflux rate and K + uptake at very low ligand concentrations, inhibition at higher levels; maximum enhancement of cation transport: (a) and (b) 10–0.1 nM (+40–50%), (c) 1–0.01 nM (2.5-fold)]. 3. 3. Binding experiments show upward-curved Scatchard graphs, with the K d values of 50 nM and 18 μM and the B max values of 10.2 and 984.5 fmol/100 μl RBC (red blood cells) respectively.
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