Abstract
Casein glycomacropeptide (GMP) is known to promote the in vitro growth of Bifidobacteria and Lactobacilli. In this paper, we used conven- tional culture techniques and fluorescent in situ hybridization (FISH) techniques to investigate the effect of casein GMP on mice fecal microbiota. The population structure of the intestinal microbiota, including Lactobacillus, Bifidobacteria, Enterococcus, coliforms and Enterobacteriaceae, was tested and compared. After consecutive administration of casein GMP for 15 days, numbers of Lactobacillus and Bifidobacteria increased significantly (P<0.01), numbers of Enterobacteriaceae and Coliforms decreased significantly (P<0.05) while no significant changes were observed for Enterococcus. The detection limits of FISH technique were significantly lower (P<0.01) than the traditional culture method. These results suggested that consumption of casein GMP had a prebiotic effect on male BALB/c mice. Casein GMP helped establish a healthier intestinal microbiota. Additionally, FISH was proved to be a rapid and relatively low-cost detection method that can be used to further our understanding of human intestinal microbiota.
Highlights
Introduction required to isolate strict anaerobes15 Such terial species that are known to be present in drawbacks have often hindered related the GIT
Lactobacillus and Bifidobacteria in the fecal sample increased after supplementing with GMP
Our study has demonstrated that use of casein GMP can significantly increase the intestinal concentration of Lactobacillus and Bifidobacteria
Summary
Introduction required to isolate strict anaerobes15 Such terial species that are known to be present in drawbacks have often hindered related the GIT. The human gut is difficult to study with probes of humans, harbor complex microorganism Recent years have seen the development of at a species level due to the vast diversity of communities. These bacterial communities new molecular techniques, especially those this ecosystem, in this study, we chose probes are comprised of up to 400 to 500 bacterial based on 16S rRNA sequences. These molecu- that are designed to recognize major genera or species forming a complex ecosystem. The techniques are important in Materials and Methods biota can help digest food, and metabolize understanding the relationship between endogenous and exogenous compounds, and microbial diversity and specific metabolic Animals and diets can potentiate the immune system and prevent activities
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