Modulation of IL-6 in ameliorating arthritic conditions in rats by Rosa alba L flower extract and fraction

Abstract

Introduction: rheumatoid arthritis characterized by joint inflammation and pain,affecting millions of peoples around the world. Traditional system of medicine hadproven to be preventive and treating physical and mental illness. Objective: theobjective of the study is to assess anti-arthritic potential of the plant Rosa alba L byconsidering CFA, formaldehyde and turpentine oil induced arthritic model. Materialand Method: ethanolic extract and its ethyl acetate fraction were considered for thestudy and quercetin was quantitatively estimated by high performance thin layer chromatographic(HPTLC) method. Moreover, hematological and biochemical studies(IL-6) were performed in blood and serum of wistar albino rat. Then histopathologicalstudies had also been performed on rat hind paw joint. Result: phytochemicalscreening estimated the presence of carbohydrates, phenolic compounds, flavonoids,phytosterols, amino acids and fixed oil in ethanolic extract and ethyl acetate fraction ofthe plant. Amount of quercetin in the flowers of the plant was found to be 0.26% w/wby quantitative HPTLC method. The level of proinflammatory cytokines, interleukin(IL-6) was considerably decreased (p<0.001) in the fraction treated group of rats at400 mg/kg b.w by considering complete freund’s adjuvant (CFA) induced arthritic model. Conclusion: Ameliorated hematological,hematological, biochemical and histopathologicalparameters confirmed the antiarthritic potential of the plant.