Abstract
Carbon nanotubes (CNTs) have wide applications in cosmetics, sunscreens and for systemic delivery of drugs and gene delivery (Aitken et al., 2006. Occup. Med. 56, 300). The toxic effects of acid functionalized single walled carbon nanotubes (AF-SWCNTs) on LA4 lung epithelial cell line (Saxena et al., 2007. Nanotoxicology 1, 291) and erythrocytes (Sachar and Saxena, 2011. PLOS ONE 6(7), e22032) have been reported. The aim of present study was to study the effect of AF-SWCNTs treatment on different aged erythrocytes and erythropoietic responses in bone marrow and spleen in C57Bl/6 mice. A newly developed double in vivo biotinylation (DIB) technique was used to assess the effect of AF-SWCNTs on different aged erythrocytes (Khandelwal and Saxena, 2006. Exp. Gerontol. 41, 855). Various stages of erythrocyte differentiation in bone marrow and spleen were enumerated by using double staining of bone marrow cells with Ter119 (Glycophorin) and CD71 (Transferrin) monoclonal antibodies followed by flow cytometric analysis (Marinkovic et al., 2007. J. Clin. Investig. 117, 2133). Mice were repeatedly treatedwith 10 gAF-SWCNTs intravenously. Our results show that AF-SWCNTs treatment induced a sustained anemia and reticulocyte production in mice. An accelerated loss of young (ranges 4–15%) and intermediate aged erythrocytes young (upto 22%) occurred at different time points. Erythroid populations in bone marrow in AF-SWCNTs treated mice show a steep fall in (upto 27%) of erythroid cells in bone marrow. Contrary to bone marrow, the proportion of erythroid cells increased significantly (upto 26%) in spleens of AF-SWCNTs treated mice.
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