Abstract
Modulation of delayed type hypersensitivity response by skin peptide from Rana tigerina
Highlights
Introduction antimicrobial property but recent findings favored it Delayed type hypersensitivity (DTH) is an antigen as a new weapon against HIV (Bradbury, 2005) and specific cutaneous reaction mediated by Th1 cells and is cancer (Baker et al, 1993)
Pathogens, including mycobacteria, fungi and certain Tripathi, 2004). These properties of frog skin peptides led parasites and may be involved in transplant rejection us to find their possible action on CMI and macrophages
Mice were sacrificed after 24 hrs and peritoneal macrophages harvested to determine the optimum dose for frog skin peptides
Summary
Peptides fractions from the HPLC separation were incubated with 100μl of suspension of bacterial strains obtained from (Gifted by IMTECH, Chandigarh) (Table 1) from log phase culture (105cells/ml) in 96 well micro titer cell culture plates for 18h at 370C in a humidified atmosphere of air bacterial strains, maintained on different nutrient media by following standard protocol. The absorbance at 630 nm of each well was determined using a micro titer plate reader
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