Abstract
ABSTRACT Bromeliads are the target of predatory extractivism and consequently many species are included in the red list of threatened species, such as those belonging to the genus Neoregelia. Although Neoregelia mucugensis has not been evaluated for the degree of threat, its exploitation is exclusively extractive and its occurrence in Chapada Diamantina-BA is subject to the action of fires that affect the region annually. In this context, applying techniques aimed at protecting this genetic resource is fundamental for both the maintenance of its natural populations and the ex situ conservation of this genetic material. Plant tissue culture techniques have been successfully applied for the conservation of several bromeliad species. One of the methods used is slow growth, which consists in reducing plant metabolism and consequently decelerating its growth, which allows the maintenance of in vitro plant collections without the need for subculture. In this context, the objective of this study was to test the reduction of salts in the culture medium and the addition of osmoregulators on the induction of slow growth of N. mucugensis. Plants were subjected to treatments composed of different concentrations of MS medium and mannitol for a period of 12 months, when then analyses were conducted to evaluate growth, chlorophyll content and regeneration capacity of shoots in vitro. It was found that the treatment containing MS ½ and 7.8 g.L-1 of mannitol is indicated for in vitro conservation of N. mucugensis with maintenance of the regenerative capacity of its tissues.
Highlights
Bromeliads are floristic representatives of marked ecological importance
Biotechnology provides successful tools such as plant tissue culture that can be used for the micropropagation and ex situ conservation of this genetic resource
Considering that studies with N. mucugensis are limited to in vitro germination and regeneration (BELLINTANI et al, 2007, 2008) and based on the hypothesis that tissue culture is an alternative for ex situ conservation, the aim of this study was to evaluate the reduction of salts and addition of osmoregulators to the medium on the induction of slow growth
Summary
Bromeliads are floristic representatives of marked ecological importance. Since the arrangement of its leaves forms ‘tanks’ that accumulate nutrient-rich water (JENSEN et al, 2011), producing ecological niches, which house fauna and flora (SCHUTTZ; ARAÚJO; SÁ, 2012). (SASAMORI; ENDRES-JUNIOR; DROSTE, 2020), using the in vitro conservation technique It can be performed by the slow growth method, which aims to increase the subculture intervals, promoting reduction of costs with reagents and labor (BELLO-BELLO; GARCÍA-GARCÍA; IGLESIAS-ANDREU, 2015). This method consists in reducing plant metabolism, commonly obtained by modifications in the chemical composition of the culture medium (SÁ; LÊDO; LÊDO, 2011), related to the removal, reduction and/or addition of substances. Considering that studies with N. mucugensis are limited to in vitro germination and regeneration (BELLINTANI et al, 2007, 2008) and based on the hypothesis that tissue culture is an alternative for ex situ conservation, the aim of this study was to evaluate the reduction of salts and addition of osmoregulators to the medium on the induction of slow growth
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