Modulation of Cellular Response to Different Parameters of the Rotating Magnetic Field (RMF)-An In Vitro Wound Healing Study.

Magdalena Jedrzejczak-Silicka,Maciej Konopacki,Marian Kordas,Rafał Rakoczy

doi:10.3390/ijms22115785

Magdalena Jedrzejczak-Silicka, Maciej Konopacki + Show 2 more

Open Access

https://doi.org/10.3390/ijms22115785

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Abstract

Since the effect of MFs (magnetic fields) on various biological systems has been studied, different results have been obtained from an insignificant effect of weak MFs on the disruption of the circadian clock system. On the other hand, magnetic fields, electromagnetic fields, or electric fields are used in medicine. The presented study was conducted to determine whether a low-frequency RMF (rotating magnetic field) with different field parameters could evoke the cellular response in vitro and is possible to modulate the cellular response. The cellular metabolic activity, ROS and Ca2+ concentration levels, wound healing assay, and gene expression analyses were conducted to evaluate the effect of RMF. It was shown that different values of magnetic induction (B) and frequency (f) of RMF evoke a different response of cells, e.g., increase in the general metabolic activity may be associated with the increasing of ROS levels. The lower intracellular Ca2+ concentration (for 50 Hz) evoked the inability of cells to wound closure. It can be stated that the subtle balance in the ROS level is crucial in the wound for the effective healing process, and it is possible to modulate the cellular response to the RMF in the context of an in vitro wound healing.

Highlights

Electromagnetobiology or electrobiomagnetism is a branch of science whose main goal is to analyze the influence of electromagnetic field (EMF) on living organisms
We sought to determine whether low-frequency rotating magnetic field (RMF) with different field parameters could evoke the cellular response in vitro (L929 and HaCaT cell culture models), and is it possible to modulate the cellular response to different parameters of the RMF in the context of an in vitro wound healing
The human keratinocytes exposed to RMF (f = 50 Hz and B = 13.6 and 28.4 mT) exhibited the lowest ability in wound healing, and those cultures displayed a lower reactive oxygen species (ROS) level and lower intracellular Ca2+ ion concentration

Summary

Introduction

Electromagnetobiology or electrobiomagnetism is a branch of science whose main goal is to analyze the influence of electromagnetic field (EMF) on living organisms. The electric and magnetic fields are components of EMF. The electric field (EF) is generated by the presence of an electric charge, and this kind of field defines the magnitude and direction of the force it exerts on a positive electric charge. The magnetic field (MF) is created by means of the motion of electric charges, and this kind of field acts only on electric charges in motion. The EMF can be organized taking into consideration their frequency or wavelength. Hunt et al (2009) showed that electromagnetic stimulation takes into account the influence of predominantly magnetic fields, predominantly electric fields, or fields with both electric and magnetic components [4]

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