Modulation of Cav1.3 Ca2+ channel gating by Rab3 interacting molecule

Abstract

Neurotransmitter release and spontaneous action potentials during cochlear inner hair cell (IHC) development depend on the activity of Cav1.3 voltage-gated L-type Ca2+ channels. Their voltage- and Ca2+-dependent inactivation kinetics are slower than in other tissues but the underlying molecular mechanisms are not yet understood. We found that Rab3-interacting molecule-2α (RIM2α) mRNA is expressed in immature cochlear IHCs and the protein co-localizes with Cav1.3 in the same presynaptic compartment of IHCs. Expression of RIM proteins in tsA-201 cells revealed binding to the β-subunit of the channel complex and RIM-induced slowing of both Ca2+- and voltage-dependent inactivation of Cav1.3 channels. By inhibiting inactivation, RIM induced a non-inactivating current component typical for IHC Cav1.3 currents which should allow these channels to carry a substantial window current during prolonged depolarizations. These data suggest that RIM2 contributes to the stabilization of Cav1.3 gating kinetics in immature IHCs.