Modulation of BTEX Bio - Remedial Activity by Molecular Triggering from Facultative Anaerobic Activity

Abstract

BTEX (Benzene, Toluene, Ethylbenzene, Xylene) is widely reside in fuel and painting process, such as industrial metal painting. Due to their volatile character, it is easily emitted and recalcitrant in disintegration through aromatic property. These vapor inhaled and slow corruption the lipid membrane of the lungs tissue. Benzene and derivatives are thus more mutagenic and carcinogenic evaluated. The leakage of oil and spoil the soil and groundwater which arouse publi c concern about health respect. Therefore, it is crucial to develop high - efficiency of pollutant transformation, with aims to instant treatment of their toxicity or start remediation process. This study had screened several active isolates from bio - fixed b iofilms consortia were carried. The investigation concerns the supplement of electronic molecules for the remediation effect of BTEX were tried and the cultivation status of facultative anaerobic state were addressed. In highlight of finding with high acti vity consortium was enriched from humic acid eluted from compost of kitchen waste, add additional 400 mg/L BTEX compound over almost one year. Active isolate were compared by using different carbon source as electron donors with 31.2 μM substrate concentra tion. After the basal carbon source as humic acid and molasses was extra added and compared, remedial activity of the strain with 3 - 3> 3 - 1> 2 - 6 were compared. As a second level analysis, the remedial effects shown: methanol>glucose>indole acetic acid>indol e, indicated that the electromotive force might influenced by the grafting carbon chain, the length of carbon grafting, the influence of reducer as ferrous ions in medium, also the free benzoic compound interacted, dynamic directing the direction of electr on flux from humic acid or molasses, overall, humic acid, generally less conversion, believed only support electron mediation in other reaction. While molasses, macromolecular and exhibits a reducing power with glucose and methanol. However, it causes inva lid electronic flux into aromatic conversion but competitively switch to oxidative respiration. Resulted an invalid co - metabolism. The significant of remedial effect by grafting aromatics and soluble humic acid were demonstrated in this report.