Abstract
<p class="Abstract">In this study, we evaluated the effects of ethanol extracts of Detam 1 soybean, Jati belanda leaf, and the combination toward expression of peroxisome proliferator-activated receptor gamma (PPARγ), CCAAT/enhancer-binding protein alpha (C/EBPα), and stearoyl-CoA desaturase 1 (SCD1) genes in 3T3-L1 cells as anti-adipogenesis and anti-obesity. The differentiation of 3T3-L1 cells into adipocyte was conducted using induction medium consist of Dulbecco's Modified Eagle's Medium, 3-isobutyl-1-methylxanthine, insulin, dexamethasone, and fetal bovine serum. The expression of PPARγ, C/EBPα, and SCD1 gene was measured using real-time quantitative polymerase chain reaction (qPCR). Ethanol extract of Jati belanda at a concentration of 50 μg/mL was most effective to reduce PPARγ, C/EBPα, and SCD1 gene expression in 3T3-L1 cells. Ethanol extract of Detam 1 soybean failed to reduce PPARγ gene expression, whilst in the concentration of 50 μg/mL it was able to significantly reduce the C/EBPα and SCD1 gene expression. Both ethanol extracts of Detam 1 soybean and Jati belanda have potential as anti-adipogenesis and anti-obesity by suppressing adipogenesis-related gene expression, particularly C/EBPα and SCD1.</p><p><strong>Video Clip:</strong></p><p><a href="https://youtube.com/v/UWv9nChmF2Y">Maintaining 3T3-L1 cell culture</a>: 5 min 15 sec</p>
Highlights
Obesity is one of the largest and fastest growing health problem both in the developed and developing countries (Sergent et al, 2012; Rodgers et al, 2012)
Our previous study demonstrated that ethanol extract of Detam 1 soybean seeds and ethanol extract of Jati belanda leaves have potential as anti-obesity, it inhibited weight gain in male Wistar rat and possessed inhibitory activities toward glucose-6-phosphate dehydrogenase (G6PD), triglyceride (TG) and cholesterol (CHOL) in 3T3-L1 cell line (Hidayat et al, 2015a; Hidayat et al, 2015b)
The quantification of peroxisome proliferator-activated receptor gamma (PPAR) gene expression using quantitative polymerase chain reaction (qPCR) revealed that only several treatments than positive control, which were ethanol extract of Jati belanda leaves (EEJB) 50 μg/mL and combination of EEJB and ethanol extract of Detam soybean (EEDS) (20 μg/mL, 10 μg/mL) (Figure 1)
Summary
Obesity is one of the largest and fastest growing health problem both in the developed and developing countries (Sergent et al, 2012; Rodgers et al, 2012). Our previous study demonstrated that ethanol extract of Detam 1 soybean seeds and ethanol extract of Jati belanda leaves have potential as anti-obesity, it inhibited weight gain in male Wistar rat and possessed inhibitory activities toward glucose-6-phosphate dehydrogenase (G6PD), triglyceride (TG) and cholesterol (CHOL) in 3T3-L1 cell line (Hidayat et al, 2015a; Hidayat et al, 2015b). We continue to study the antiadipogenesis properties of ethanol extract of Detam 1 soybean (EEDS) and ethanol extract of Jati belanda leaves (EEJB) in 3T3-L1 cell line by analyzing the expression of PPAR and C/EBP genes which are important transcription factor in adipogenesis (Rosen et al, 2002) and SCD1 gene which plays a role in lipid storage in adipocytes (Ralston and Mutch, 2015)
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