Modulation of a Specific Pattern of microRNAs, Including miR-29a, miR-30a and miR-34a, in Cultured Human Skin Fibroblasts, in Response to the Application of a Biofunctional Ingredient that Protects against Cellular Senescence &amp;lt;i&amp;gt;in Vitro&amp;lt;/i&amp;gt;

Xianghong Yan,Catherine Serre,Valère Busuttil,Ludivine Mur,Jean-Marie Botto,Laurine Bergeron,Nouha Domloge

doi:10.4236/jcdsa.2015.54040

Modulation of a Specific Pattern of microRNAs, Including miR-29a, miR-30a and miR-34a, in Cultured Human Skin Fibroblasts, in Response to the Application of a Biofunctional Ingredient that Protects against Cellular Senescence &lt;i&gt;in Vitro&lt;/i&gt;

Xianghong Yan, Catherine Serre + Show 5 more

Open Access

https://doi.org/10.4236/jcdsa.2015.54040

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Abstract

Skin aging is a process of structural and compositional remodeling that can be manifested by wrinkling and sagging. Remarkably, the dermis plays a dominant role in the aging process. Recent studies suggest that microRNAs are implicated in the regulation of gene expression during aging. However, studies about age-related microRNAs and how they modulate skin aging remain limited. In the present work, a complex of hydrolyzed natural yeast proteins (Saccharomyces cerevisiae) and hydrolyzed natural soya bean was developed and showed the ability to modulate the expression of telomere-binding protein TRF2, which is a key factor for telomere protection and to prevent cellular senescence in vitro and DNA damage. The aim of the study was to identify microRNAs specifically modulated after application of the ingredient complex to cultured fibroblasts, and their possible involvement in remodeling of the human extracellular matrix and fibroblast senescence. Consequently, human skin fibroblasts were cultured and treated with 1% of the ingredient complex for 48 h before analyzing microRNA modulation by RT-qPCR. The use of bioinformatics allowed us to predict the target genes for modulated microRNAs. Results show that the ingredient complex modulated a pattern of microRNAs including the down-regulation of miR-29a-3p, miR-30a-5p and miR-34a-5p, which are associated with fibroblast senescence and remodeling of the human dermal extracellular matrix. In conclusion, our results indicate that miR-29a-3p, miR-30a-5p and miR-34a-5p possibly represent key microRNAs that impact human fibroblast senescence and remodeling of the dermal extracellular matrix.

Highlights

The skin is the largest organ of the human body and fulfills many important functions
The purpose of the present study was to identify microRNAs involved in fibroblast senescence and modulation of the dermal extracellular matrix structure, and to identify potentially modulated target genes by bioinformatic prediction
Modulation of the expression level of the microRNAs on this list was assessed by microRNA qPCR array, in cultured normal human fibroblast treated or not with the natural ingredient complex

Summary

Introduction

The skin is the largest organ of the human body and fulfills many important functions. It plays a vital role in protecting the internal organs from external aggression and environmental factors such as trauma, ultraviolet and infrared radiation, heat, environmental pollution and microbial pathogens. The dermis plays a dominant role in the process of skin aging. While this process is subject to both intrinsic and extrinsic factors, the consequences involve abnormal synthesis of proteins in the dermal extracellular matrix, increased degradation of collagen and elastin, and senescence of dermal fibroblasts

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