Abstract
In recent days, cheapest alternative carbon source for fermentation purpose is desirable to minimize production cost. Xylanases have become attractive enzymes as their potential in bio-bleaching of pulp and paper industry. The objective of the present study was to identify the potential ability on the xylanase production by locally isolated Bacillus pumilus BS131 by using waste fiber sludge and wheat bran media under submerged fermentation. Culture growth conditions were optimized to obtain significant amount of xylanase. Maximum xylanase production was recorded after 72 hours of incubation at 30 °C and 7 pH with 4.0% substrate concentration. In the nutshell, the production of xylanase using inexpensive waste fiber sludge and wheat-bran as an alternative in place of expensive xylan substrate was more cost effective and environment friendly.
Highlights
Agro-industrial waste is among the major environmental pollutants
Further increase of fermentation period resulted in a decline of enzyme production which might be due to the production of some toxic metabolites during microbial growth which inhibits the enzyme synthesis
Kumar and Satyanarayana (2014) reported 56 h of fermentation period was optimum for xylanase production by an alkaliphilic Bacillus pumilus VLK-1 in submerged fermentation conditions (SmF) using wheat bran as a substrate
Summary
Agro-industrial waste is among the major environmental pollutants. Their microbial conversion is a solution to many environmental problems and a source of suitable byproducts like food, fuel and chemicals (Milala et al, 2005). Wheat bran is one of the most abundant agricultural wastes of Pakistan food industries whereas fiber sludge is paper industry waste of no value. Microbial xylanases from high yielding strains of Aeromonas, Agrobacterium, Bacillus, Dictyoglomus, Nocardia, Pseudomonas, Thermotoga and Xanthomonas are grown under submerged fermentation conditions (SmF). Studies have shown that some nutritional factors including the sources of carbon and nitrogen can influence the production of xylanase enzyme (Farjalla et al, 2006). Some physical factors such as time course study, temperature, pH, substrate
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