Modulated cementogenic genes upregulation in human buccal fat pad-derived stem cells by strontium-ranelate

Abstract

Cementogenesis plays a pivotal role in some critical clinical complications including replantation of avulsed teeth. Strontium-ranelate has been shown to affect bone regeneration. Despite the resemblance of bone and dental cementum, there are a few studies regarding the impact of strontium-ranelate on cementogenic differentiation. Current study aimed to investigate the proliferation and cementoblastic differentiation of human buccal fat pad-derived stem cells (BFPSCs) while treated by strontium-ranelate in comparison to its osteogenic differentiation. To do this, BFPSCs were treated with different concentrations of strontium ranelate (0, 6, 12.5, 25, 50, 100 and 200 μM). MTT assay, Alizarin red staining, and real-time RT-PCR were performed to evaluate cementoblastic and osteoblastic markers. The highest expression of cementoblast markers including: cementum protein-1 and cementum attachment protein were observed at concentrations of 50 and 100 μM after 7 and 14 days of induction. The results demonstrated that strontium-ranelate could dose-dependently affect the proliferation and cementoblastic differentiation of BFPSCs. Taking together, strontium-ranelate could be used in combination with BFPSCs to improve root resorption following orthodontic therapy.