Modified vegetables extract as substitution of v8-juice medium for cultivation of Phytophthora spp

Abstract

Phytophthora species is a pathogen that causes rot on plant parts both on roots, stems, leaves and fruit. One obstacle in investigating the mechanism of infection and virulence testing of Phytophthora palmivora is to obtain suitable media for its growth. The purpose of this research was to study the suitability of modified vegetables extract as substitution of V8 juice for growth medium of Phytopthora spp. This research consisted of several stages, which were, preparation of V8-Juice medium and vegetable medium, isolation of P. palmivora and P. colocasia from infected cacao pod and taro, and observation of P. palmivora and P. colocasiae by their morphological characters. The results showed that this medium was effective for cultivation of P. palmivora and P. colocasiae. There was no morphological difference was observed in the growth of P. palmivora both on V8-Juice medium and on vegetable extract medium. The appearance of hyphal swelling and characteristics of sporangium, oospore, chlamydospore and zoospore observed have the same characteristics in both types of media used. Modified vegetable media was also effective for cultivation of taro leaf blight pathogen P. colocasiae.