Abstract
A modified tetrazolium-based colorimetric assay was used to determine the anti-HIV activities of ddAzThd, ddCyd, ddIno, and PFA. In this assay, poly-1-lysine-coated plates were used to attach the MT-2 cells to the bottom of the plates. A fixed amount of virus (50 TCID 50) was used in each well. A modified version of the formula published by Pauwels et al. (1988) was used for calculating the percentage cell protection from virus infection. Using CC10/ EC90 to calculate the selective indices, the decreasing order of selectivity against HIV-1 strain A87SF, was: ddAzThd > PFA > ddCyd > ddIno. Against HIV-1 strain A79SK-1 the decreasing order of selectivity was: PFA > ddIno > AzThd > ddCyd. The modified formula showed lack of antiHIV activity for thymidine at non-toxic concentrations.
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