Abstract
AbstractAn original concept in technology of biosensitive electrodes based on single‐wall carbon nanotubes (SWCNT) is proposed. According to a newly proposed technique the electrode surface consisting of SWCNT’s embedded in a porous polycarbonate film was preoxidized enzymatically using laccase from Basidiomycete Lac. An efficient electron transfer from the active centre of PQQ‐dependent glucose dehydrogenase (s‐PQQ‐GDH) to the SWCNT electrode surface allowed the application of the s‐PQQ‐GDH as recognition element for the reagentless biosensor design. The residual activity of the biosensor was found to be 40 % after one month of operational working. A mathematical model of the biosensor has been developed and an admissible agreement between the simulation results and the experimental data was obtained. The model appears to be promising for optimization of amperometric analysis.
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