Abstract

We describe a simple and practical approach to the modified Steiner procedure for demonstrating spirochetes and nonfilamentous bacteria. The silver impregnation is done in a microwave oven and results in slender black organisms against a pale yellow background. Advantages of the method include shorter reducing times, elimination of nonselective precipitation, and diminished cellular debris. The method works in the conventional oven as well. (The J Histotechnol 10:241, 1987.)

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