Modified single node culture method—a new micropropagation method for chickpea

Abstract

Chickpea was micropropagated by axillary shoot proliferation (ASP) and modified single node culture (MSNC) methods. Maximum propagule proliferation occurred on Murashige and Skoog (MS) medium enriched with 1–10 µM N6-benzyladenine and 0.01 µM α-naphthaleneacetic acid. The propagules were rooted on MS medium containing 1 µM 3-indolebutyric acid and B5 vitamins. Regenerated plants were fertile and phenotypically similar to control plants grown from seed. The MSNC method was four times more efficient than the ASP method in terms of the number of plants produced per explant.