Abstract
Folic acid (FA) as one of the water-soluble vitamins contributes to the construction of healthy cells, as FA deficiency is one of the leading causes of anaemia. Based on reports, reduced folate level can lead to development of carcinogenesis. Hence, its analysis from the clinical point of view is highly demanded. In the present work, NiFe2O4 nanoparticles was successfully synthesized and used for modified novel voltammetric sensor for determination of folic acid. Differential pulse voltammetry response shows the linear increment of oxidation signals with an increase in the concentration of folic acid in the range of 1.0 × 10-7-5.0 × 10-4 M with limit of detection 3.4 × 10-8 M. The modified electrode displays an excellent selectivity towards the determination of FA even in the presence of various interfering species. Finally, the screen printed electrode (SPE) consists of three main parts which are a graphite counter electrode, a silver pseudo-reference electrode and a graphite working electrode modified by NiFe2O4 nanoparticles (NFO) was applied for FA determination folic acid tablet and urine samples whose accuracy was attested by means of addition and recovery assays (97.0-103.5%) as well as by differential pulse voltammetry.
Highlights
Application of screen-printed electrodes (SPEs) has a main advantage of miniaturization compared to the conventional electrodes including carbon paste or glassy carbon electrodes.[1]
As can be seen from Fourier transform infrared (FT-IR) spectra the normal mode of vibration of tetrahedral cluster (599 cm–1) is higher than that of octahedral cluster (465 cm–1). This is due to the shorter bond length of tetrahedral cluster than the octahedral cluster. bye-Scherrer formula as 40.0 nm. t = 0.9 λ / β cos (θ) where λ is the wavelength of the X-ray radiation (1.54056 Å for Cu lamp), θ is the diffraction angle and β is the full width at half-maximum (FWHM). 30
The results show that the electrocatalytic peak currents of folic acid oxidation at the surface of NFO/ screen printed electrode (SPE) were linearly dependent on folic acid concentrations over the range of 1.0–500.0 μM, while the detection limit (3σ) was obtained as 0.023 μM
Summary
Application of screen-printed electrodes (SPEs) has a main advantage of miniaturization compared to the conventional electrodes including carbon paste or glassy carbon electrodes.[1]. The mixed solutions, with stoichiometric 30 molar ratio of urea/Fe3+ (with excess urea that form sufficient precipitating ions for metal oxides formation), were magnetically stirred until all the starting materials were totally dissolved at 25 °C. These solutions were further homogenized in an ultrasonic water bath for 15 min and respectively transferred into Teflon-lined stainless steel autoclave with a capacity of 200 mL in order to keep them at 200 °C for 24 h in an oven. The resulting products were dried in a vacuum oven at 105 °C for 12 h
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