Abstract
In order to analyze the mycotoxins in corn, the modified QuEChERS high-performance liquid chromatography was applied to extract, clean up, and detect mycotoxins in a nonpolar system. The impurities such as fat and protein were removed from the corn sample, and the impurities in the sample solution have almost no effect on extraction efficiency. The proposed method leads to a greater choice of mycotoxin-extraction solvents in high-fat-solid samples. An appropriate extraction solvent was selected, the pretreatment conditions were optimized, and the extraction and cleanup of mycotoxins in high-fat solids were enhanced. By changing the experiment parameters, this method can be further used for the extraction and analysis of mycotoxins in complex samples (nonfat, low-fat, or high-fat). This method achieves good linearity in the range of 2.5–1000 μg/kg with the correlation coefficients for all analytes in the range of 0.9975 to 0.9989. The acceptable standard deviations for intraday and interday precision were 1.8–4.3% and 3.2–5.2%, respectively, with recoveries from 89.7 to 105.9%.
Highlights
In 1960, about 100,000 turkeys died from acute aflatoxin poisoning in the United Kingdom
Mycotoxins are a series of toxic secondary metabolites produced by some molds after they mature [3,4,5]. ey can be divided into field toxins and storage toxins, depending on where they grow [6, 7]. e identification and classification of molds have traditionally been based on morphological and cultural characteristics, but the morphological characteristics of molds are complex, and morphological studies of them are often questioned on the basis of lack of standards and excessive subjectivity. e common mycotoxin is aflatoxins (AFs, including aflatoxin B1 (AFB1), aflatoxin B2 (AFB2), AFG1, and AFG2), zearalenone (ZEN), deoxynivalenol (DON), sterigmatocystin (ST), citreoviridin (CIT), ochratoxin A (OTA), and so on [8]
After 30 s, the tube was vigorously shaken for 5 min and the 6 mL extraction solvent and 0.3 g NaCl were added into the tube. e ultrasonic extraction was carried out for 5 min, the mixed solution was centrifuged at 0°C and 15,000 rpm for 5 min, and the supernatant was transferred into another centrifuge tube containing 80 mg of adsorbent (40 mg of diatomaceous earth + 40 mg PSA); after shaking, the mixture was centrifuged at 15,000 rpm for 5 min, the supernatant was transferred to a glass flask and evaporated to dryness at 35°C in a vacuum rotary evaporator, and the residue was redissolved with 100 μl of solvent
Summary
In 1960, about 100,000 turkeys died from acute aflatoxin poisoning in the United Kingdom. E common mycotoxin is aflatoxins (AFs, including AFB1, AFB2, AFG1, and AFG2), zearalenone (ZEN), deoxynivalenol (DON), sterigmatocystin (ST), citreoviridin (CIT), ochratoxin A (OTA), and so on [8]. China’s national standards stipulate that the MRLs of AFB1, DON, ZEN, and OTA in corn are 20, 1000, 60, and 5 μg/kg, respectively (GB 2761-2017). In the European Union, the MRLs of AFB1, DON, ZEN, and OTA in corn are 2.0, 1750, 350, and 5 μg/kg, respectively. In the U.S, the MRLs of AFB1, DON, ZEN, and OTA in corn are 5.0, 4000, 350, and 5 μg/kg, Journal of Chemistry respectively. Erefore, it is necessary to continuously improve the relevant analysis methods, which will help to come up with a more reasonable limit standard to ensure the corn food security The classification of standard is still not specific enough to effectively distinguish the limit value of raw grain and finished grain [13,14,15]. erefore, it is necessary to continuously improve the relevant analysis methods, which will help to come up with a more reasonable limit standard to ensure the corn food security
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
