Abstract
This protocol outlines and evaluates a modified scanning procedure for a customized spectral domain optical coherence tomography (SD-OCT) imaging apparatus within the wild-type C57Bl/6 mouse posterior segment. This modified protocol allows for the capture of a 50 degree field of view spanning 3 mm by 3 mm perimeter with the optic disc as the central point. By utilizing this scanning protocol a more reliable measurement of retinal thickness can be achieved outside the fluctuating region of the optic disc. This protocol, when applied to this high resolution device, enables non-invasive in vivo histological imaging and biometric assessment of the various layers of the rodent posterior segment within a 20 – 30 min procedural time-frame. This protocol could establish a standardized method for evaluating morphological changes, with this commercial SDOCT device, when assessing longitudinal disease pathophysiology and treatment response in mouse models for future vision science research.
Highlights
Mouse models represent valuable paradigms for studying retinal neurodegenerative and vascular disorders that are analogous in humans
Mouse models have been instrumental for understanding pathophysiology in a variety of retinal diseases including: retinopathy of prematurity [1], diabetic retinopathy [2,3,4], exudative age-related macular degeneration [5], retinal vascular occlusion and ischemia-reperfusion injury models [6] and inherited retinal diseases [7,8,9,10,11]
We describe a modified scanning protocol for a stereotactic rotational multidirectional animal containment apparatus, with spectral domain optical coherence tomography (SD-OCT) imaging capabilities, for the acquisition of high resolution scans of the ocular posterior segment in mice
Summary
Mouse models represent valuable paradigms for studying retinal neurodegenerative and vascular disorders that are analogous in humans. The gold standard approach for studying retinal disease changes in these animal models has been through the use of ex vivo histological preparations after sacrificing the animal. This methodology is fraught with limitations as it provides one time observation and includes tissue damage, toxicity, laborious technical procedures, as well as tangential assessment of disease pathophysiology. In vivo techniques such as fundoscopy, confocal scanning laser ophthalmoscopy (cSLO), angiography, and electroretinography (ERG) allow longitudinal observation of dynamic functional and morphological changes within disease models, but do not provide histological evidence
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