Modified graphene-based nanocomposite material for smart textile biosensor to detect lactate from human sweat

Abstract

Hydrophobic cotton fabric and chemically modified G-PU-RGO-PB paste based electrochemical wearable lactate biosensor provides on body monitoring for patient. Sensor contains three electrodes system designed by template method where working electrode (WE) and counter electrodes (CE) filled with modified G-PU-RGO-PB paste and Ag/AgCl were used to fill the reference electrode. In addition, embroidered conductive silver yarn work as a conductor was used to secure connection between printed electrodes and the device. Here, we present a suitable technique to get stable electron transfer catalytic activity while enzyme was immobilized over the working electrode. Electrode's performance was examined, using cyclic voltammetry technique (CV) in ferricyanide solution (5.0 mM-25mM), and found linear relationship with relative standard deviations (RSD) ∼0.2%. Lactate oxidase and chitosan solution was combined to the electrode surface for amperometric and differential pulse voltametric sensing. The limit of detection (LOD) of 0.4 mM and the limit of quantification (LOQ) of 1.3 mM for lactate was obtained from human sweat, respectively. This sensor gives excellent reproducibility to the lactate concentration after washing the sensor by domestic washing machine and its RSD was found 3.06%. We anticipate that the results, which have been achieved after analysis, would open up the fundamental studies in the area of wearable textile-based sensor for real time health care monitoring and medical diagnosis.