Modifications of nuclear envelope during differentiation and depolyploidization of rat trophoblast cells

Abstract

An increased activity of membranes of the nuclear envelope (NE) was observed electron microscopically in the trophoblast cells of the rat placenta. The activity of the membranes was manifested as formation of various NE derivatives, such as the annulate lamellae (AL), the intranuclear tubules, and the concentric membranous structures. At the period of terminal differentiation of the secondary giant trophoblast cells (SGTC) the NE derivatives play active role in subdivision of the initial highly polyploid nuclei into the numerous low-ploidy fragments. 3H-thymidine labeling showed that attenuation of the DNA replication precedes the nuclear fragmentation. In the course of the nuclear fragmentation the narrow deep NE invaginations subdivide the nucleus into the separate lobes that subsequently are detached from the initial nucleus. By the beginning of the fragmentation, the accumulated membranous structures, i.e. the intranuclear AL, tubules, clusters of pore complexes, etc., seem to be the source of the reserve material that is necessary for formation of the great amount of the NE membranes of the newly formed nuclear fragments. Thus, the intranuclear membranous structures that seem to increase the active surface of the growing endopolyploid nucleus at the earlier stage of differentiation then take part in genome isolation that results in formation of a multinucleate cell with diploid and low-polyploid nuclei. The outer NE membrane of the initial nucleus plays an active role in compartmentalization of cytoplasmic areas around the nuclear fragments within the giant polykaryocyte. Apart from the membranous structures the bundles of intermediate filaments (IF) located in the cytoplasm perinuclear zones seem to participate in the nuclear fragmentation. These processes are likely to provide formation of the giant polykaryocytes incapable for further proliferation.