Modifications and in vitro absorption of 5-heptadecyresorcinol from cereals using digestion and ussing chamber models

Abstract

5-Heptadecylresorcinol (AR-C17), a homologue of alkylresorcinols (ARs) and mainly observed in cereal brans, has stronger physiological functions compared with its homologues. However, not only is its content rare but also the purification low. Besides, few researches on its digestion characteristics and bioavailability limits its maximum applications. Here, we mainly relied on solid-state fermentation, embedment, in vitro models to systematically evaluate processing technologies, digestion and absorption characteristics of AR-C17. We showed that the highest content of AR-C17 was 57.6 μg/g extracted from triticale bran fermented by Saccharomyces cerevisiae relying on ultrasound-assistance. Additionally, AR-C17 was chiefly absorbed in duodenum and jejunum, and its apparent absorption increased by around 2.1 times when quercetin was added as the synergistic agent, which was higher than other phenolics in bran extract. Furthermore, AR-C17 embedded by β-cyclodextrin avoided the decomposition of in strong acidic environment, enhancing the retention rate to 96 % in in vitro digestion. According to the results above, we mixed AR-C17 with the quercetin, and embedded the mixture by β-cyclodextrin, which maximized the apparent absorption of AR-C17, reaching 19.8 % when the ratio of quercetin and AR-C17 was 1:1.