Abstract
Abstract The USP XVI chemical method for vitamin D in USP products has been substantially modified and applied to evaporated milk containing approximately 30 units of vitamin D per fluid ounce. A major modification is a 15 second reading at 500 mμ which most nearly represents the vitamin D content of the sample. An improved sample preparation eliminates much of the background interference, which also produces a color reaction. This method, when applied to a variety of samples, will show a relative standard deviation of 10%. Statistical data are presented to compare the modified method with the rat bioassay method for assaying vitamin D in evaporated milk.
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