Abstract
Significant mercury inhibitory effects on photosystem II (PSII) in algal cells and thylakoid membranes were previously reported. The inhibition of PSII activity is consequent to a mercury effect on the PSII oxidizing side at the water-splitting system (1–4). Earlier, we indicated that the mercury quenching effect on variable fluorescence is related to the inactivation of some PSII reaction centers due to the loss of the functional interaction between the water-splitting system and the PSII reaction centers (4). Further study of mercury fluorescence quenching requires new parameters which are necessary to describe the mechanisms of the PSII inhibition by mercury. Recently, the Stern-Volmer method was shown to be suitable to provide useful parameters for the understanding of chlorophyll fluorescence quenching mechanisms in chloroplasts (5,6). In this report, we modified the Stern-Volmer method to analyze the mercury fluorescence quenching effet in the green alga Dunaliella tertiolecta.
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