Abstract
Eight hays (two each of alfalfa, bahiagrass, bermudagrass, and digitgrass) were used in three experiments to evaluate effects on neutral detergent fiber of 1) eliminating sodium sulfite and decalin from the refluxing reagent, 2) replacing sintered glass crucibles during filtration with Gooch crucibles containing glass wool mats, and 3) eliminating final acetone washes of residues. Sodium sulfite and decalin should be eliminated from the refluxing reagent. Sodium sulfite often decreased neutral detergent fiber by important amounts (more than 3%). Decalin may cause significant over-estimation of neutral detergent fiber. Decalin is not required as an antifoam agent if rate of boiling is controlled during refluxing. Gooch crucibles (40ml) with mats of glass wool should replace sintered glass crucibles for recovery of residues. Sintered glass crucibles may cause significant overestimation of neutral detergent fiber when sodium sulfite is eliminated from the refluxing reagent. Filters of sintered glass crucibles may lose mass when residues are ashed. Final acetone washes of residues should be continued until possible interactions among acetone washes, crucible type, and hay are investigated.
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