Abstract
The effects of two extractants on the activities of carboxypeptidase and proteinase enzymes from three sorghum varieties germinated for 3 and 5 days were studied. In all three varieties, cysteine hydrochloride (Cyst. HCl) proved a better extractant than 2-mercaptoethanol (2-ME) as shown by the increase in carboxypeptidase and proteinase activities with 5-day malts. A three-way analysis of variance (ANOVA) on the effect of germination time, extractant and variety on the carboxypeptidase activities of the three malts showed that all the factors plus their possible four-way interactions highly significantly (P < 0.001) affected the extractability/activity of the enzyme. However, while the three factors had a highly significant (P < 0.001) effect on the proteinase activity, one of their four-way interactions, specifically, extractant/variety, had no significant effect. This suggests that, unlike the carboxypeptidase enzyme, the effectiveness of the extractants in enhancing the extractability/activity of the proteinase enzyme in all the sorghum malt samples was amongst other factors, not variety-dependent.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
