Abstract
I.p. immunization with gamma-irradiated hepatoma cells induces resistance to s.c. tumour-cell challenge in syngeneic WAB/Not rats. Mild heat treatment of these cells (greater than 41 degrees C for 30 min) destroyed this immunoprotective effect, but did not abolish tumour-specific antibody production in treated rats. The binding of syngeneic and alloantibodies to surface antigens expressed on hepatoma cells was unaffected by heat treatment. Thus, heat-treated gamma-irradiated hepatoma cells retain a serologically defined tumour-specific antigen but are unable to elicit immunoprotection. By examining the incorporation of radioactive precursors into DNA, RNA and protein in heat-treated cells, it was determined that above 41 degrees C there was a significant decrease in metabolic activity. It is postulated that for the effective induction of transplantation immunity to tumours, tumour-specific antigens should be present on the surface membranes of a metabolically active cell. This hypothesis accounts for the absence or marked reduction of immunoprotection induced by inviable or glutaraldehyde-treated cells, isolated cell membranes and soluble tumour extracts which retain serologically defined tumour-specific antigens.
Highlights
Summary.-I.p. immunization with y-irradiated hepatoma cells induces resistance to s.c. tumour-cell challenge in syngeneic WAB/Not rats
To evaluate whether the retention of a residual metabolic activity in y-irradiated cells was associated with their immunogenic capacity, these cells were incubated at various elevated temperatures for 30 min
Since the results of this communication indicate that tthe immunogenicity of y-irradiated D23 cells against viable cell challenge is associ,ated with the retention of residual metabtolic activity, and since the previous report demonstrated that immunogenicity was labile to glutaraldehyde treattment (Price et al, 1979), the effects of glutaraldehyde upon the metabolic activity oif y-irradiated cells was examined
Summary
Summary.-I.p. immunization with y-irradiated hepatoma cells induces resistance to s.c. tumour-cell challenge in syngeneic WAB/Not rats. Heat-treated y-irradiated hepatoma cells retain a serologically defined tumour-specific antigen but are unable to elicit immunoprotection. It is postulated that for the effective induction of transplantation immunity to tumours, tumour-specific antigens should be present on the surface membranes of a metabolically active cell. This hypothesis accounts for the absence or marked reduction of immunoprotection induced by inviable or glutaraldehyde-treated cells, isolated cell membranes and soluble tumour extracts which retain serologically defined tumour-specific antigens. There is evidence that for the induction report (Price et al, 1979) it was deterof a primary lymphoid cell-mediated mined that glutaraldehyde stabilization of immune response in vitro, active metabo- hepatoma D23 cell surfaces abolished lism of the stimulating cells is required their immunogenicity, even though sero-
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