Modification of the Friberg microagglutination tray technique for detection of sperm antibodies.

Abstract

The Friberg microagglutination tray technique for the detection of sperm-agglutinating antibodies in the serum has several advantages over other techniques. Microquantities of a single-donor sperm specimen may be used for several tests and the agglutinates may be examined directly with an inverted lense microscope. However, in its original form, there were a few technical problems. The technique has been modified to eliminate the problems. The donor sperm is filtered through a glass wool filter. The procedure was adapted for Terasaki tissue culture plates. With these modifications the technique seems more practical for clinical usage.