Abstract
Suitable buffer additives can either induce or reduce the electrophoretic mobility of certain polysaccharides during capillary electrophoresis. With water-soluble cellulose derivatives, adsorption of detergents on the analyte molecules governs the migration rates. Different types of adsorption sites, including multilayer formation, were found to be present on the cellulose-derived polymers. For highly charged polysaccharides (heparins), the electrophoretic mobility can be controlled by the use of ion-pairing reagents. The concentration and number of cationic charges appear useful in optimizing the separation selectivity. Migration models based on secondary thermodynamic equilibria are presented and evaluated for different polysaccharides.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
