Modification of the effects of aflatoxin B1 on the glutathione system and its regulatory genes by zeolite.

Benjámin Kövesi,Mátyás Cserháti,Márta Erdélyi,Szabina Kulcsár,Krisztián Balogh,Erika Zándoki,Zsolt Ancsin,Miklós Mézes

doi:10.1556/004.2021.00002

Abstract

The purpose of the present study was to use oxidative stress markers for investigating the effect of zeolite (315 mg/kg of complete feed) in the case of aflatoxin B1 contamination (92 μg/kg complete feed). In a 21-day feeding trial with broiler chickens, oxidative stress parameters such as conjugated dienes, conjugated trienes, malondialdehyde, reduced glutathione content and glutathione peroxidase activity were not changed significantly by supplementation with this mycotoxin absorbent. The relative gene expression of transcription factors KEAP1 and NRF2 was not modified by the absorbent either. Still, the expression of GSS, GSR and GPX4 genes increased significantly due to the aluminosilicate supplementation. The results suggest that zeolite reduced lipid peroxidation in the blood plasma but not in the red blood cell haemolysate or the kidney. The relative expression of the genes encoding the glutathione redox system also changed as a result of zeolite supplementation, but these changes were not found at the protein level.

Highlights

Aflatoxins (AFs), produced primarily by Aspergillus flavus and Aspergillus parasiticus, are unavoidable natural contaminants of feedstuffs and basic foods (Abrar et al, 2013)
The results suggest that zeolite reduced lipid peroxidation in the blood plasma but not in the red blood cell haemolysate or the kidney
The average daily aflatoxin B1 (AFB1) intake calculated from the feed intake was nearly the same in the groups fed aflatoxincontaminated or aflatoxin-contaminated and mycotoxin absorbent containing diets (Table 3)

Summary

Introduction

Aflatoxins (AFs), produced primarily by Aspergillus flavus and Aspergillus parasiticus, are unavoidable natural contaminants of feedstuffs and basic foods (Abrar et al, 2013). Aflatoxin contamination in complete feeds in Europe showed high variation during the last decade. Between 2006 and 2007, the rate of AFB1-. Positive feed samples was 8%, with 47 mg/kg as mean and 311 mg/kg as highest contamination (Binder et al, 2007). Between 2009 and 2011, the rate of AFB1-positive feed samples was. 24.5%, with 3.5 mg/kg being the mean and 52 mg/kg the highest values (Rodrigues and Naehrer, 2012). In 2019, the rate of samples positive for AFB1 was 8%, with 10 mg/kg as the mean and 237 mg/kg as the highest concentrations (BIOMIN, 2020)

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