Abstract
Freezing in liquid nitrogen does not result in appreciable modification of the activities of hydrolases or peptidases in human sperm. In contrast the activities of 6-P-gluconate dehydrogenase and, particularly, of glucose 6-phosphate dehydrogenase are severely diminished or even abolished after freezing and thawing. This is not the result of a denaturation of the enzymes, but rather a leak into the surrounding medium. These results are discussed with reference to the role of the pentose pathway in the energy metabolism of human sperm; this enzyme leakage from the spermatozoon could constitute a reliable test assay for evaluation of cell damage due to deep freezing.
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