Abstract

Summary The effect of exogenous application of gibberellic acid, indole-3-acetic acid and 1-ascorbic acid on the free nucleotide pool, the content and synthesis of RNA and total acid RNase was investigated in dark-germinating barley. Estimations made at different stages of growth on various organs revealed that while the degree and direction of the effect of each growth regulator were organ specific and, at times, sequential in nature, their action on RNase was similar. Ascorbic acid induced the maximum enhancement of RNase activity in the embryonic axis (ca. 150%), compared with the effect of IAA and GA (ca. 50% and 30% enhancement respectively). GA did not induce any significant stimulation of RNA synthesis, whereas IAA and AA did so in the case of the shoot.

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