Modification of reversed-phase liquid chromatographic retention characteristics for metal—tetraphenylporphyrin complexes using octane as a mobile phase additive

Abstract

The effect of the addition of octane to a methanolic mobile phase on the reversed-phase liquid chromatographic separation of a series of porphyrin compounds is described. The capacity factors of tetraphenylporphyrin (TPP) and five metal—TPP complexes decreased with increase in the octane content of the mobile phase. Selective sorption of octane on the surface of ODS occurred. The addition of octane to the mobile phase caused an alteration in the sequence of elution, improved the resolution of some TPP complexes and reduced the time required for analysis.