Modification of QuPPe-PO-Method for the Determination of Glyphosate and Aminomethylphosphonic Acid using High Strength Silica Chromatographic Column

Abstract

Glyphosate (Gly) is a popular herbicide often used for crop desiccation. The use of glyphosate leads to the contamination of the agricultural products as well as soil, surface and groundwater. For food safety control and environment monitoring, a method developed based on liquid chromatography tandem mass spectrometry, which allows quantitative determination of glyphosate and aminomethylphosphonic acid (AMPA), without derivatization. The LOQ of Gly and AMPA in raw materials of plant origin is 0.1 mg/kg; LOQ of Gly in water is 0.001 mg/L, AMPA is 0.002 mg/L; LOQ of Gly in soil is 0.02 mg/kg, AMPA is 0.04 mg/kg. Extraction from raw materials of plant origin was performed by acetic acid solution of methanol in water, in the presence of EDTA-Na2 and dichloromethane. Extraction from water was carried out in the presence of EDTA-Na2 and acetic acid. From soil, Gly and AMPA were extracted with 0.1% of ammonia solution. The extracts are purified on OASIS HLB; proteins were precipitated with acetonitrile, an aliquot of the extract was concentrated. The resulting calibration dependence equations were linear and the correlation values (R) were ≥ 0.99. Relative standard deviation values were in the range of 1.77-19.5%, while the recoveries were from 80.5 to 108%.