Modification of platelet adherence to protein-coated surfaces.

Abstract

Abstract Washed pig platelets labeled with 14 C-serotonin were used to study platelet adherence to glass surfaces coated with albumin or fibrinogen and the platelet release reaction induced by a gamma globulin-coated surface. Platelets adhered to the fibrinogen-coated surface without undergoing the release reaction. Both adherence to the fibrinogen-coated surface and release were diminished by inhibition of glycolysis with iodoacetate; a combination of iodoacetate and antimycin caused almost complete inhibition. Adherence to the fibrinogen-coated surface and release were also inhibited by chelation of divalent cations and by agents (prostaglandin E 1 , caffeine, and dibutyryl cyclic AMP) that increase platelet cyclic AMP. Of the pyrimidopyrimidine compounds tested (RA8, RA233, and RA433) only RA433 inhibited both adherence and release. Acetylsalicylic acid, phenylbutazone, and sulfinpyrazone inhibited both adherence to the fibrinogen-coated surface and release induced by the gamma globulin-coated surface. The nonsteroidal anti-inflammatory drugs and sulfinpyrazone may exert some of their inhibitory effect on the release reaction through their inhibition of the adherence of platelets to surfaces.