Abstract
The plastic Cooper-type of dish has been adapted to high power, high resolution phase-contrast microscopy by drilling a 3/4 inch hole in the center of the bottom of the dish and cementing a 30 × 30 mm coverslip over this hole. Once the trypsinized cell suspension has been added and the dish closed, the top of the dish is sealed to the bottom by means of a roll of plasticene to prevent loss of CO2 and water. The preparation is maintained in a stage incubator. The same field can be observed with an inverted phase contrast microscope for 3 to 4 days. Either high dry (40-45x) or oil immersion objectives may be used to study the cells. The high resolution obtainable is especially useful in evaluating the effects of viruses or chemical agents on living cells in monolayer cultures.
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