MODIFICATION OF GELATIN/CARBONATED HYDROXYAPATITE MEMBRANE WITH CHITOSAN TO IMPROVE THE TENSILE STRENGTH

Abstract

Membrane for guided tissue regeneration (GTR) is an interesting method for treating periodontitis cases. Synthesis of membranes for GTR based on gelatin and carbonated hydroxyapatite (CHA) modified by chitosan using the freezedrying method has been successfully carried out. This study aims to obtain the optimum composition between gelatin and chitosan to obtain an effective combination as a membrane base material. The membrane was synthesized using a ratio of gelatin (G) and chitosan (CH) = 1:0, 1:0.5, 1:1, 1:1.5, 1:2 and 1:2.5. The addition of chitosan to gelatin and CHA is intended to improve the physical properties of gelatin to increase membrane stability, both in dry and wet conditions. The synthesized membranes were characterized using SEM-EDX, FTIR, XRD, tensile strength test, and water absorption test. The results of the tensile strength test showed that the membrane with a ratio of G: CH = 1:1 had good mechanical strength. It was indicated by the data of the highest tensile strength compared to other compositions. The SEM-EDX characterization results show that the membrane with the composition G: CH = 1:1 had a fairly hollow surface texture. It allows it to enter the water to have adequate absorption capacity. The absorption capacity of the membrane with a composition of G: CH = 1:1 was not much different from membranes with other compositions, while XRD data showed insignificant differences in crystallinity between membrane material compositions. The FTIR spectra all showed the typical adsorption bands of gelatin and chitosan as well as the formation of hydrogen bonds and electrostatic forces between gelatin and chitosan. The composition of G: CH = 1:1 proved to be more effective as a membrane material composition with the highest tensile strength, namely 1.34 ± 0.07 MPa (dry) and 0.06 ± 0.01 MPa (wet), and an adsorption capacity of 1486 ± 109%