Abstract
ObjectiveTo generate Candida antarctica lipase A (CAL-A) mutants with modified fatty acid selectivities and improved lipolytic activities using error-prone PCR (epPCR).ResultsA Candida antarctica lipase A mutant was obtained in three rounds of epPCR. This mutant showed a 14 times higher ability to hydrolyze triacylglycerols containing conjugated linoleic acids, and was 12 and 14 times more selective towards cis-9, trans-11 and trans-10, cis-12 isomers respectively, compared to native lipase. Lipolytic activities towards fatty acid esters were markedly improved, in particular towards butyric, lauric, stearic and palmitic esters.ConclusionDirected molecular evolution is an efficient method to generate lipases with desirable selectivity towards CLA isomers and improved lipolytic activities towards esters of fatty acids.
Highlights
Directed molecular evolution is used for improving or altering the activity of enzymes
Electronic supplementary material The online version of this article contains supplementary material, which is available to authorized users
Directed molecular evolution may result in the disadvantageous changes in the protein structure due to the random nature of mutations, and the large libraries of mutants may result in the poor chances of obtaining enzyme with the desired properties (Chica et al 2005)
Summary
Directed molecular evolution is an efficient method to generate lipases with desirable selectivity towards CLA isomers and improved lipolytic activities towards esters of fatty acids. Electronic supplementary material The online version of this article (doi:10.1007/s10529-017-2299-0) contains supplementary material, which is available to authorized users. D. Głod Department of Pathophysiology, University of Warmia and Mazury in Olsztyn, al.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
