Modification of crystallinity and crystalline structure of Acetobacter xylinum cellulose in the presence of water-soluble β-1,4-linked polysaccharides: 13C-NMR evidence

Abstract

Cellulose produced by Acetobacter xylinum in medium containing 0.5% xyloglucan or glucomannan showed altered crystallinities and shifted I α I β ratios when analysed by solid-state 13C-NMR. By estimating the spectra of cellulose components in each composite, a decreased I α content was shown to be countered by increased I β content in cellulose aggregated in the presence of xyloglucan, causing minimal loss of crystallinity. However, the I α decrease was linked primarily to increased disordered content in cellulose produced in medium containing glucomannan. These results are considered in the light of two models for the morphological disposition of the I α phase: (i) a series model, proposed on the basis of electron diffraction measurements for an algal cellulose, in which regions of I α and I β alternate along the length of a microfibril, and (ii) a superlattice model, in which the I α and I β domains co-exist throughout the cross-section of each microfibril and form as a result of hierarchical aggregation. The latter model offers clearer insight into the role of the polysaccharides in inhibiting the formation of I α crystalline regions. In this superlattice model, polysaccharides adsorbed on surfaces of the most elementary aggregates are displaced to varying degrees during subsequent aggregation, with the presence of these polysaccharides altering the extent of I α production at interfaces.