Abstract

Rapid and simple modifications of conventional high-performance liquid chromatography equpment enabled use of the system for carbon dioxide supercritical fluid chromatography. No permanent alteratinos were required, and the sytem could be used interchangeably for conventional or supercritical fluid chromatographh. The system described was capable of operating in four elution modes: (1) isocratic—isobaric; (2) solvent gradient; (3) flow gradient; (4) combined solvent—flow gradient. Each elution mode was successfully used for the separation of a polynuclear aromatic hydrocarbon mixture. Effects of system parameters, such as pressure, temperature, and flow-rate were also examined.

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