Modification of α-amylase from Bacillus licheniformis by the polyaldehyde derived from β-cyclodextrine and α-amylase thermostability

Abstract

The cleavage of β-cyclodextrine by sodium periodate at the seven 2–3 diols of the glucose unit gives rise to the polyaldehyde 1, used to modify α-amylase. The reductive modification of α-amylase from Bacillus licheniformis reduced the number of reactive lysine groups from 8 to 3.5 per mol of enzyme with an activity loss of 25% and increased the half-life at 80°C from 4.7 to 7.0 minutes.