Abstract
Flavonoids, specialized metabolites found in plants, have a number of beneficial properties and are important for maintaining good health. Efficient extraction methods are required to extract the most bioactive compounds from plant material. Modern techniques are replacing conventional methods of flavonoids extraction in order to reduce energy and solvent consumption, increase extraction efficiency, and satisfy growing market demand as well as environmental legislation. The extraction of bioactive molecules compounds is affected by a number of variables. To determine the conditions that ensure the highest extraction yield, it is advisable to analyze the interactions between the above in parallel. In this work, an overview of the advantages and performance of modern methods (microwave-assisted extraction, ultrasound-assisted extraction, pressurized liquids-assisted extraction, and supercritical fluids extraction) for the extraction of flavonoids is presented. This work also presents the application of extraction process optimization and extraction kinetics for flavonoid extraction, using different types of experimental designs for different flavonoid sources and different extraction methods. The general conclusion of all the studies listed is that an experimental design combined with RSM modeling reduces the number of experiments that should be performed to achieve maximum extraction yield.
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