MODERN MOLECULAR BIOLOGICAL TYPES OF ENDOMETRIAL CANCER: COMPARATIVE ENDOCRINE AND PROINFLAMMATORY-PROGENOTOXIC CHARACTERISTICS

Abstract

Aims were to compare the characteristics of hormonal and metabolic status, a set of proinflammatory and antiinflammatory markers, as well as factors associated with DNA damage/ genome integrity in endometrial cancer (EC) patients, divided on the basis of the contemporary classification that distinguishes four molecular-biological types of this tumor. Materials and methods: The study involved material from untreated EC patients, the number of which varied depending on the method used. The average age of patients was close to 60 years and about 90% of them were in postmenopausal period. In the course of evaluation of pro-inflammatory and antiinflammatory factors (levels of cytokines IL-6 and TNF, myo-kine myostatin and adipokine prefilin / Pref-1 /, the ratio of neutrophils/lymphocytes in the blood), an enzyme immunoassay and hematological analysis were used, while the study of the expression of lipoxygenases’ mRNA (“pro-inflammatory” alox5 and “anti-inflammatory” alox15) in omental adipose tissue was based on real-time PCR methodology. Besides, the comet tail moment and % of DNA in the tail of comets of mononuclear cells, the level of 8-hydroxy-2-deoxyguanosine in circulation and the telomere length of mononuclear cells were, respectively, measured as an indicators of DNA damage or genome integrity. Results and conclusions: According to the data obtained, the differences between EC patients are determined not only by the propensity for overweight or lack of it, but also by belonging of the individual case to one or another molecular-biological type established on the basis of genetic and immu-nohistochemical analysis of the tumor tissue. Altogether this may indicate certain specificity of stimuluses leading to the development of particular endometrial cancer variant.