Abstract

This study has the aim to realise the karyotype in dogs using new and original techniques of staining, reading and realizing of the karyogram. To fulfill the proposed goal we used the classical visualization techniques of the chromosomes in dog lymphocytes. We analyzed both metaphases and chromosomes in G banding. The Karyotype was realized using a motorized Zeiss Axioscop 2 microscope having a capture camera connected with a computer with a special software dedicated for the chromosomes karyotyping – MetaSystem Ikaros. The software is normally used to karyotype the human chromosomes, but it was adapted by us to karyotype the chromosomes in dog. Compared to the classical karyotyping methods, this software is very facile and for this reason we recommend it to be used to realize the karyotype in animals

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