Abstract
The review describes recent approaches used for evaluation of cytochrome P450 concentration and activity. It considers employment of modern methods of proteomic analysis including electrophoresis and chromatography/mass-spectrometry (MS) for investigation of the particular group of proteins. Special attention is paid to targeted quantitative MS analysis of cytochromes P450 in biological samples. Finally, we analyze methods used for assay of cytochromes P450 activities and problems of correlation between content of certain P450 isoforms and their specific enzymatic activities.
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Schedule a callMore From: Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry
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