Modern diagnosis of IgE-mediated allergy – molecular diagnosis of allergies

Abstract

Diagnostic difficulties resulting from the imperfections of natural allergen extracts inspired to use genetic engineering techniques to produce recombinant allergens or obtaining highly purified components (component) allergen. This led to the development of modern diagnostic technique in allergy or molecular diagnostics. The basis for understanding the molecular diagnosis of allergies is to know the properties of allergens. Each allergen is composed of various proteins known. component capable of sensitizing allergen, and each component includes a plurality of epitopes that can be divided into one species-specific epitopes, and the identical amino acid structure of the epitopes derived from different species. Specific epitopes are responsible for primary sensitization, while the epitopes with similar structures are responsible for cross-reactions. Finding sensitization several epitopes is a strong indication of the occurrence of much more dangerous allergic reactions than only one epitope. In addition, molecular diagnosis of allergies allows for personalized diagnosis of allergic patients. It enables the assessment of individual risk of allergic symptoms and allows you to distinguish the original from allergy symptoms caused by cross-reactions. It should be noted, however, that the diagnosis of allergy should be based on a comprehensive evaluation of the results and their confrontation with data from the interview. The mere detection of allergen-specific IgE antibodies, even the method of molecular diagnostics, without the presence of clinical symptoms does not confirm an allergy or illness. Only goes to confirm that the body of such a person is allergic and that the symptoms of this condition may at some point reveal but not necessarily.