Modern approaches to the application of photoimmunomodulating technologies in the treatment of HPV-associated cervicitis

Abstract

We aimed to investigate the effects of the combined use of polychromatic visible and infrared polarized light with topical application of Imiquimod 5% cream on the macrophage link of immunity in patients with chronic human papillomavirus (HPV)-associated cervicitis.
 Materials and methods. The study included 90 patients aged 2035 years, who had a history of HPV-associated cervicitis for at least 1 year. Patients were distributed into three groups by random sampling. The main group included 30 patients who were exposed to blue monochromatic polarized incoherent light on the projection of the carotid arteries and polychromatic visible and infrared polarized light on the uterine cervix in combination with topical application of Imiquimod 5% cream (complex one). The comparison group included 30 patients who were exposed to polychromatic visible and infrared polarized light on the uterine cervix in combination with Imiquimod 5% cream (complex 2), and the control group included 30 patients who received local exposure to the uterine cervix with Imiquimod 5% cream. Examination data of 20 healthy women of the same age were taken as normal values. The macrophage link activity was assessed using the nitroblue tetrazolium test.
 Results. Under the influence of the developed complexes 1 and 2, the percentage of phagocytic cells of the macrophage link increased significantly to normal values (by 36% and 31%, respectively; p 0.01), the phagocytic number and index of phagocytosis completeness increased to near-normal values (p 0.001). In addition, the percentage of spontaneous (excessive) redox processes of neutrophils decreased from 21.1% 1.3% to 11.8 1.01% in the main group (p 0.001) and to 14.4 1.5% in the comparison group (p 0.01). Less significant results were obtained in the control group where the patients received topical application of Imiquimod 5% cream on the uterine cervix, and the studied parameters were 26%51.7% lower than that in the main group.
 Conclusion. The complex application of blue monochromatic polarized incoherent light on the projection of the carotid arteries in combination with polychromatic visible and infrared polarized light and external application of Imiquimod 5% cream on the cervix of patients with chronic HPV-associated cervicitis has a pronounced immunocorrecting effect on the immunity macrophage link. This is confirmed by an improvement in the indicators of the functional activity of neutrophils, in the form of a decrease in the percentage of excessive redox reactions and an increase in the index of phagocytosis completeness, and indicates an increase in the productivity of the macrophage link functioning.